Preanalytical evaluation of serum 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 measurements using LC–MS/MS
نویسندگان
چکیده
منابع مشابه
Assay for plasma 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 by "high-performance" liquid chromatography.
We describe a new, rapid, and simple column-chromatographic procedure for 25-OH vitamin D3 in plasma. The vitamin is extracted by use of Sep-pak C18 (octadecyl alkylated silicic acid), a short factory-packed reversed-phase column, and 25-OH vitamin D2 + 25-OH vitamin D3 fraction is eluted with methanol/water. The 25-OH D2 and 25-OH D3 are then well resolved on a high-resolution 3-micrometer sil...
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BACKGROUND The relative potency of 25-hydroxyvitamin D3 to vitamin D3 needs to be better defined so that food-composition tables can better reflect the true vitamin D nutritive value of certain foods. OBJECTIVE We performed a randomized, controlled intervention study in apparently healthy, free-living adults to investigate whether the intake of 25-hydroxyvitamin D3 is 5 times more potent in r...
متن کاملSerum 25-hydroxyvitamin D3 and 24R,25-dihydroxyvitamin D3 concentrations in adult dogs are more substantially increased by oral supplementation of 25-hydroxyvitamin D3 than by vitamin D3
We previously found a weak response in serum 25-hydroxyvitamin D3 (25(OH)D3) concentrations when dogs were supplemented with oral vitamin D3 (D3). In the present study, we determined the relative potency of oral 25(OH)D3 compared with D3 for increasing vitamin D status in dogs with low serum 25(OH)D concentrations. Four male and three female, 4-year-old, intact, lean, genetically related, Chine...
متن کاملSPE and LC-MS/MS Method for the Determination of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 from Human Plasma
A liquid chromatography tandem mass spectrometry method for 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 from human plasma has been developed. Sample preparation was fast and efficient using a Thermo ScientificTM SOLATM hydrophobic reversed phase (HRP) plate. Analysis was carried out using a Thermo ScientificTM SyncronisTM C18 1.7 μm, 50 × 2.1 mm column to give a fast separation with a cycle t...
متن کاملDevelopment and optimization of an LC-MS/MS-based method for simultaneous quantification of vitamin D2 , vitamin D3 , 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3.
Simultaneous and accurate measurement of vitamin D and 25-hydroxyvitamin D in biological samples is a barrier limiting our ability to define "optimal" vitamin D status. Thus, our goal was to optimize conditions and evaluate an LC-MS method for simultaneous detection and quantification of vitamin D(2) , vitamin D(3) , 25-hydroxyvitamin D(2) and 25-hydroxyvitamin D(3) in serum. Extraction and sep...
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ژورنال
عنوان ژورنال: Clinica Chimica Acta
سال: 2013
ISSN: 0009-8981
DOI: 10.1016/j.cca.2012.10.041